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pspax2 second generation lentiviral packaging plasmid  (Addgene inc)


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    Addgene inc pspax2 second generation lentiviral packaging plasmid
    Pspax2 Second Generation Lentiviral Packaging Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+generation+lentiviral+packaging+plasmid+pspax2/psPAX2+(Plasmid+%2312260)/bio_rxiv__64898__2026__03__26__714479-230-7-13
    Average 98 stars, based on 15695 article reviews
    pspax2 second generation lentiviral packaging plasmid - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Expressing:

    Article Title: Potent 3CLpro inhibitors effective against SARS-CoV-2 and MERS-CoV in animal models by therapeutic treatment
    Article Snippet: .. Lentivirus-based pseudoviruses expressing SARS-CoV-2 Wuhan-Hu-1 strain were generated with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Watertown, MA), a reporter plasmid pUCGFP-Luc (Addgene), and pAbVec-SARS2-S ( ). .. Both plasmids expressing hACE2 or TMPRSS2 were obtained from Origen (Rockville, MD).

    Article Title: Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expression
    Article Snippet: .. HEK 293 T cells were transfected with second-generation lentiviral packaging plasmid psPAX2 (Addgene, # 12260), VSV-G envelope expressing plasmid pMD2.G (Addgene, # 12259) and transfer plasmid (FUdeltaGW-rtTA, FUW-tetO-YFP, FUW-tetO-YFP-Tat, FUW-tetO-YFP-Zta, FUW-tetO-YFP1-Tat-P2A-YFP2-Zta) at 15:6:20 ratio using Turbofect transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions (20 μg of plasmid DNA and 40 μl of Turbofect transfection reagent were premixed in 2 ml of OptiMEM medium (Gibco) and added to 20 ml of DMEM/10% FBS cell medium in T-175 flask). ..

    Article Title: Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells
    Article Snippet: The next day, HEK293T cells were transfected using the JetPRIMETM transfection reagent (PEQLAB) following the manufacturer’s recommendations. .. Transfection was carried out using 1.5 μg of the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260), 0.5 μg of the envelope expressing plasmid pMD2.G (Addgene, 12259) and 2 μg of the cloned expression vector, encoding for the gene of interest. ..

    Article Title: Histone binding of ASF1 is required for fruiting body development but not for genome stability in the filamentous fungus Sordaria macrospora .
    Article Snippet: .. Lentivirus-based pseudoviruses expressing SARS-CoV-2 Wuhan-Hu-1 strain were generated with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Watertown, MA), a reporter plasmid pUCGFP-Luc (Addgene), and pAbVec-SARS2-S (42). .. Both plasmids expressing hACE2 or TMPRSS2 were obtained from Origen (Rockville, MD).

    Generated:

    Article Title: Potent 3CLpro inhibitors effective against SARS-CoV-2 and MERS-CoV in animal models by therapeutic treatment
    Article Snippet: .. Lentivirus-based pseudoviruses expressing SARS-CoV-2 Wuhan-Hu-1 strain were generated with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Watertown, MA), a reporter plasmid pUCGFP-Luc (Addgene), and pAbVec-SARS2-S ( ). .. Both plasmids expressing hACE2 or TMPRSS2 were obtained from Origen (Rockville, MD).

    Article Title: Histone binding of ASF1 is required for fruiting body development but not for genome stability in the filamentous fungus Sordaria macrospora .
    Article Snippet: .. Lentivirus-based pseudoviruses expressing SARS-CoV-2 Wuhan-Hu-1 strain were generated with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Watertown, MA), a reporter plasmid pUCGFP-Luc (Addgene), and pAbVec-SARS2-S (42). .. Both plasmids expressing hACE2 or TMPRSS2 were obtained from Origen (Rockville, MD).

    Plasmid Preparation:

    Article Title: Potent 3CLpro inhibitors effective against SARS-CoV-2 and MERS-CoV in animal models by therapeutic treatment
    Article Snippet: .. Lentivirus-based pseudoviruses expressing SARS-CoV-2 Wuhan-Hu-1 strain were generated with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Watertown, MA), a reporter plasmid pUCGFP-Luc (Addgene), and pAbVec-SARS2-S ( ). .. Both plasmids expressing hACE2 or TMPRSS2 were obtained from Origen (Rockville, MD).

    Article Title: Critical role for a high-plasticity cell state in lung cancer.
    Article Snippet: .. HEK293FreeStyle (HEK293FS) cells were transfected with lentiviral transfer plasmids and the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260) and the envelope plasmid pMD2.G (Addgene, 12259) using either the TransIT-LT1 kit (Mirus Bio, MIR 6000) or Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, 11668500). ..

    Article Title: Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expression
    Article Snippet: .. HEK 293 T cells were transfected with second-generation lentiviral packaging plasmid psPAX2 (Addgene, # 12260), VSV-G envelope expressing plasmid pMD2.G (Addgene, # 12259) and transfer plasmid (FUdeltaGW-rtTA, FUW-tetO-YFP, FUW-tetO-YFP-Tat, FUW-tetO-YFP-Zta, FUW-tetO-YFP1-Tat-P2A-YFP2-Zta) at 15:6:20 ratio using Turbofect transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions (20 μg of plasmid DNA and 40 μl of Turbofect transfection reagent were premixed in 2 ml of OptiMEM medium (Gibco) and added to 20 ml of DMEM/10% FBS cell medium in T-175 flask). ..

    Article Title: Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells
    Article Snippet: The next day, HEK293T cells were transfected using the JetPRIMETM transfection reagent (PEQLAB) following the manufacturer’s recommendations. .. Transfection was carried out using 1.5 μg of the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260), 0.5 μg of the envelope expressing plasmid pMD2.G (Addgene, 12259) and 2 μg of the cloned expression vector, encoding for the gene of interest. ..

    Article Title: Histone binding of ASF1 is required for fruiting body development but not for genome stability in the filamentous fungus Sordaria macrospora .
    Article Snippet: .. Lentivirus-based pseudoviruses expressing SARS-CoV-2 Wuhan-Hu-1 strain were generated with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Watertown, MA), a reporter plasmid pUCGFP-Luc (Addgene), and pAbVec-SARS2-S (42). .. Both plasmids expressing hACE2 or TMPRSS2 were obtained from Origen (Rockville, MD).

    Article Title: Critical role for a high-plasticity cell state in lung cancer
    Article Snippet: .. HEK293FreeStyle (HEK293FS) cells were transfected with lentiviral transfer plasmids and the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260) and the envelope plasmid pMD2.G (Addgene, 12259) using either the TransIT-LT1 kit (Mirus Bio, MIR 6000) or Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, 11668500). ..

    Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma
    Article Snippet: MRTX1133 (a kind gift from Mirati Pharmaceuticals) in captisol (#HY-17031, MedChem Express) at 30 mg/kg, b.i.d. .. HEK293FreeStyle (HEK293FS) cells were transfected with lentiviral transfer plasmids and the second-generation lentiviral packaging plasmid psPAX2 (Addgene, #12260) and the envelope plasmid pMD2.G (Addgene, #12259) via either the TransIT-LT1 kit (Mirus Bio, #MIR 6000) or Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, #11668500). ..

    Article Title: A UBH-UBX module amplifies p97/VCP’s unfolding power to facilitate protein extraction and degradation
    Article Snippet: The targeting sequence for FAF2 (5’-tgaggagcgggatctaaccc-3’) was cloned into the LentiCRISPR v2 plasmid (Addgene, Cat#52961). .. The plasmid was co-transfected into HEK293T cells with the second-generation lentiviral packaging plasmid psPAX2 (Addgene, Cat52961) and envelope-expressing plasmid pMD2.G (Addgene, Cat#12259). .. After 48 h, puromycin (1 μg/ml, Thermo Fisher Scientific) was added for positive selection.

    Transfection:

    Article Title: Critical role for a high-plasticity cell state in lung cancer.
    Article Snippet: .. HEK293FreeStyle (HEK293FS) cells were transfected with lentiviral transfer plasmids and the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260) and the envelope plasmid pMD2.G (Addgene, 12259) using either the TransIT-LT1 kit (Mirus Bio, MIR 6000) or Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, 11668500). ..

    Article Title: Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expression
    Article Snippet: .. HEK 293 T cells were transfected with second-generation lentiviral packaging plasmid psPAX2 (Addgene, # 12260), VSV-G envelope expressing plasmid pMD2.G (Addgene, # 12259) and transfer plasmid (FUdeltaGW-rtTA, FUW-tetO-YFP, FUW-tetO-YFP-Tat, FUW-tetO-YFP-Zta, FUW-tetO-YFP1-Tat-P2A-YFP2-Zta) at 15:6:20 ratio using Turbofect transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions (20 μg of plasmid DNA and 40 μl of Turbofect transfection reagent were premixed in 2 ml of OptiMEM medium (Gibco) and added to 20 ml of DMEM/10% FBS cell medium in T-175 flask). ..

    Article Title: Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells
    Article Snippet: The next day, HEK293T cells were transfected using the JetPRIMETM transfection reagent (PEQLAB) following the manufacturer’s recommendations. .. Transfection was carried out using 1.5 μg of the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260), 0.5 μg of the envelope expressing plasmid pMD2.G (Addgene, 12259) and 2 μg of the cloned expression vector, encoding for the gene of interest. ..

    Article Title: Critical role for a high-plasticity cell state in lung cancer
    Article Snippet: .. HEK293FreeStyle (HEK293FS) cells were transfected with lentiviral transfer plasmids and the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260) and the envelope plasmid pMD2.G (Addgene, 12259) using either the TransIT-LT1 kit (Mirus Bio, MIR 6000) or Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, 11668500). ..

    Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma
    Article Snippet: MRTX1133 (a kind gift from Mirati Pharmaceuticals) in captisol (#HY-17031, MedChem Express) at 30 mg/kg, b.i.d. .. HEK293FreeStyle (HEK293FS) cells were transfected with lentiviral transfer plasmids and the second-generation lentiviral packaging plasmid psPAX2 (Addgene, #12260) and the envelope plasmid pMD2.G (Addgene, #12259) via either the TransIT-LT1 kit (Mirus Bio, #MIR 6000) or Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, #11668500). ..

    Clone Assay:

    Article Title: Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells
    Article Snippet: The next day, HEK293T cells were transfected using the JetPRIMETM transfection reagent (PEQLAB) following the manufacturer’s recommendations. .. Transfection was carried out using 1.5 μg of the second-generation lentiviral packaging plasmid psPAX2 (Addgene, 12260), 0.5 μg of the envelope expressing plasmid pMD2.G (Addgene, 12259) and 2 μg of the cloned expression vector, encoding for the gene of interest. ..



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